Optimizing Deep Learning Models for Cell Recognition in Fluorescence Microscopy: The Impact
of Loss Functions on Performance and Generalization
Luca Clissa, Antonio Macaluso, and Antonio Zoccoli
Lecture Notes in Computer Science – Submitted on Jul 2023
In the rapidly evolving domain of fluorescence microscopy, the application of deep learning techniques
for automatic cell segmentation presents exciting opportunities and challenges. In this work, we
investigate the impact of loss functions and evaluation metrics on model performance and generalization
in the context of cell recognition. First, we present extensive experiments with different commonly
used loss functions and offer practical insights and guidelines, underscoring how the choice of a
loss function can influence model performance. Second, we conduct a detailed examination of several
evaluation metrics with their relative benefits and drawbacks, helping to guide effective model evaluation
and comparison in the field. hird, we discuss how characteristics specific to fluorescence microscopy
data impact model generalization. Precisely, we examine how factors such as cell sizes, color irregularities,
and textures can potentially affect the performance and adaptability of these models to new data.
Collectively, these insights provide a nuanced understanding of deep learning for automatic cell
segmentation, shedding light on best practices, evaluation strategies, and model generalization.
We hope this study can serve as a beneficial resource for researchers and practitioners working
on similar applications, fostering further advancements in the field.